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| Registros recuperados: 174 | |
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| Barreto,A.M.E.C.; Takei,K.; E.C.,Sabino; Bellesa,M.A.O.; Salles,N.A.; Barreto,C.C.; Nishiya,A.S.; Chamone,D.F.. |
| We compared the cost-benefit of two algorithms, recently proposed by the Centers for Disease Control and Prevention, USA, with the conventional one, the most appropriate for the diagnosis of hepatitis C virus (HCV) infection in the Brazilian population. Serum samples were obtained from 517 ELISA-positive or -inconclusive blood donors who had returned to Fundação Pró-Sangue/Hemocentro de São Paulo to confirm previous results. Algorithm A was based on signal-to-cut-off (s/co) ratio of ELISA anti-HCV samples that show s/co ratio ³95% concordance with immunoblot (IB) positivity. For algorithm B, reflex nucleic acid amplification testing by PCR was required for ELISA-positive or -inconclusive samples and IB for PCR-negative samples. For algorithm C, all... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis C virus; Immunoblot; ELISA; Polymerase chain reaction; Algorithm; Diagnosis. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000200009 |
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| Binaghi,Maria Julieta; Greco,Carola Beatriz; Martín,María Eugenia; Drago,Silvina Rosa; Ronayne de Ferrer,Patricia Ana; López,Laura Beatriz. |
| Se evaluó la detección de trazas de soja en sistemas modelo (SM) de galletitas y extrudidos que contenían 5000, 250, 100, 50, 25, 10, 5 y 0 ppm de concentrado de proteína de soja (CPS). Se analizaron, además, dos lotes diferentes de ocho clases de galletitas comerciales y dos tipos de extrudidos que declaraban la presencia de soja a través de la frase “Contiene ...” o a través de frases de advertencia. La determinación de proteínas de soja se realizó utilizando tres kits ELISA (R-Biopharm, Neogen-Veratox y Romer). Solo el kit de R-Biopharm permitió la cuantificación de soja a partir de 25 ppm de CPS en galletitas y a partir de 5 ppm de CPS en productos extrudidos. Los otros dos kits resultaron menos sensibles. De acuerdo con los... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alergenos; Soja; Galletitas; Productos extrudidos; ELISA. |
| Ano: 2017 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572017000400011 |
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| Maciel,João Leodato Nunes; Duarte,Valmir; Silveira,José Ricardo Pfeifer. |
| A ocorrência de populações latentes de Ralstonia solanacearum em plantas de batata (Solanum tuberosum) pode representar fonte de inóculo de potencial desconhecido. Além disso, também é desconhecido se a população latente da bactéria é menor em cultivares resistentes do que em cultivares suscetíveis. Com a finalidade de estudar estes aspectos, um experimento foi conduzido a campo em dois locais no Estado do Rio Grande do Sul, Brasil. O objetivo foi verificar se havia relação entre a densidade populacional e o grau de resistência de cultivares de batata. O experimento foi conduzido em Eldorado do Sul, durante o período de primavera, e em Caxias do Sul, durante o período de outono. Tubérculos das cultivares Achat, Baronesa, Elvira, Macaca, Monte Bonito e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Murcha bacteriana; Solanum tuberosum; ELISA; Imunofluorescência. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000100004 |
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| Gouvêa,R.; Santos,F.F.; Machado,L.S.; Panzenhagen,P.H.N.; Aquino,M.H.C.; Nascimento,E. Rosendo do; Ribeiro,C.; Pereira,V.L.A.. |
| A enrofloxacina é um dos antimicrobianos mais utilizados na avicultura industrial, e a deposição de resíduos em produtos avícolas, como os ovos, são de grande importância para a saúde pública. Na legislação brasileira não existe padronização do período de carência para o seu uso na produção avícola e não há Limite Máximo de Resíduo (LMR) fixado para enrofloxacina em ovos. Neste estudo, foi utilizado o kit de ELISA comercial (Bioo Scientific(r)) e a LC-MS/MS na pesquisa de enrofloxacina em ovos de 30 galinhas tratadas previamente via água de bebida, com 10mg/kg de enrofloxacina, durante cinco dias. Seis ovos foram coletados diariamente e analisados durante o tratamento e após a sua suspensão, durante 15 dias. A deposição de resíduos obteve níveis máximos no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enrofloxacina; Resíduos; Ovos; LC-MS/MS; ELISA. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601917 |
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| Sepúlveda R.,Paulina; Morales,Francisco; Castaño,Mauricio. |
| En los últimos años se han observado plantas de frejol (Phaseolus vulgaris) con síntomas característicos de una enfermedad viral. Durante las temporadas 1993/1994 y 1995/1996 se realizaron prospecciones de campos de frejol en la zona productora (V a la VIII Región) de Chile. Se colectaron un total de 173 muestras de plantas afectadas por diferentes síntomas de posible etiología viral. Las plantas fueron sometidas a la prueba de ELISA con un antisuero para el Virus del Mosaico de la Alfalfa (AMV). Se determinó que era el AMV, el cual estaba distribuido en el área productora de frejol de Chile, pero con una frecuencia de detección relativamente baja, del 2 y 4% respectivamente, para las temporadas mencionadas. El procedimiento descrito junto con la... |
| Tipo: Journal article |
Palavras-chave: AMV; Aislamiento de virus; ELISA. |
| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072001000300012 |
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| Álvarez,J; Miguel Cotes,J; Marín,M. |
| La virosis del tomate de árbol en Colombia es causada por un complejo de al menos siete virus, que incluyen miembros de los géneros Potyvirus, Cucumovirus, Polerovirus y Tobamovirus, entre otros. La propagación de este frutal se realiza por semilla sexual, por lo cual es fundamental garantizar su sanidad, especialmente de virus como PVY y PLRV, que han sido informados y pueden ser transmitidos por esta vía. En la investigación se realizaron los análisis filogenéticos de aislados de PVY y PLRV obtenidos a partir de muestras de semilla sexual y plántulas de vivero de tomate de árbol procedentes de diferentes regiones de Colombia. También se desarrollaron técnicas de hibridización molecular con sondas dirigidas al gen de la cápside, como herramientas de... |
| Tipo: Journal article |
Palavras-chave: ELISA; Hibridización molecular; PLRV; PVY; Solanum betaceum. |
| Ano: 2011 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522011000200003 |
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| NICKEL,OSMAR; FAJARDO,THOR V. M.; ARAGÃO,FRANCISCO J. L.; CHAGAS,CÉSAR M.; KUHN,GILMAR B.. |
| An isolate of Grapevine virus B (GVB), obtained by indexing Vitis labrusca and V. vinifera grapevines on the indicator LN33, was transmitted mechanically to several Nicotiana species. The virus was partially purified from N. cavicola and the coat protein estimated at 23 kDa by SDS-PAGE. In negatively stained leaf extracts of experimentally inoculated N. cavicola and N. occidentalis, flexuous particles with cross banding were observed, predominantly measuring 750-770 x 12 nm, with a modal length of 760 nm. Decoration indicated a clear, positive reaction against AS-GVB. In DAS-ELISA, GVB was detected in N. cavicola and grapevine extracts, and Western blots showed homologous and cross reaction of GVB and GVA antisera with GVB coat protein. Using specific... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GVB; Vitis spp.; Vitivirus; Electron microscopy; ELISA; Western blot; GVA. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000300007 |
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| Barbosa da Silva,Jenevaldo; Nunes de Santana Castro,Gustavo; Nunes dos Santos,Priscilla; Henrique da Fonseca,Adivaldo; Henrique da Silva Lima,Danillo; dos Anjos Bomjardim,Henrique; dos Santos Belo Reis,Alessandra; de Oliveira Soares,Susiane; Diomedes Barbosa,José. |
| The aim of this study was to determine the prevalence of Toxoplasma gondii in cattle from the Northern and Midwestern regions of Brazil. Serum samples were collected from 1789 animals and tested by indirect enzyme-linked immunosorbent assay (ELISA). The overall prevalence of T. gondii was 83.40% (1492/1789). The prevalence rates of T. gondii-seropositive animals observed in the states of Pará, Tocantins and, Mato Grosso were 87.45%, 87.79% and 73.06%, respectively. The detection of high prevalence rates of T. gondii in cattle deserves special attention because they are the main source of high biological value protein for humans. This finding indicates the need for further studies on the risk that these animals may pose to public health. |
| Tipo: Journal article |
Palavras-chave: Cattle; ELISA; IFAT; Toxoplasma gondii. |
| Ano: 2015 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2015000100008 |
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| Wesongah, J. O.; Murilla, G. A.; Kibugu, J. K.; Jones, T. W.. |
| Twenty eight sheep and 28 goats were treated with isometamidium chloride (Samorin®, hone Merieux, Lyon, France) at a dose of 1 mg/kg body weight (bw) by intramuscular injection. All the animals were grazed in a tsetse-infested area. They were monitored for anaemia, body weight, anti-trypanosome antibodies and serum isometamidium concentration using the isometamidium enzyme-linked immunosorbent assay (ELISA) for more than 80 days after treatment. Serum isometamidium levels were higher in goats than in sheep throughout the experimental period. Isometamidium was still detectable in sheep and goats for up to 77 and 98 days, respectively, alter treatment (detection limit=0.1 ng/ml). The isometamidium elimination half-lives in sheep and goats were approximately... |
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Palavras-chave: Isometamidium chloride; ELISA; Sheep; Goats; Trypanosomosis. |
| Ano: 2000 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/132 |
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| Bannai, Hiroshi; Nishikawa, Yoshifumi; Seo, Jin-yong; Nakamura, Chinatsu; Zhang, Sofa; Kimata, Isao; Takashima, Yasuhiro; Li, Junyou; Igarashi, Ikuo; Xuan, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南. |
| An enzyme-linked immunosorbent assay (ELISA) based on the recombinant p23 of Cryptosporidium parvum was established for the detection of antibodies against C. parvum in cattle. The sensitivity and specificity of the ELISA were evaluated with the standard indirect fluorescent antibody test (IFAT) using sporozoites as antigens. Of 77 bovine sera collected from China, 20 (26.0%) were identified as positive by the IFAT. The same samples were tested with the ELISA. The optical densities at 415 nm were compared to the IFAT results and statistically analyzed to designate a provisional cut-off point. As a result, the cut-off point was concluded to be 0.08, which was considered to be the best condition in the light of its sensitivity (80%) and specificity (73.7%).... |
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Palavras-chave: Cryptosporidium parvum; P23; ELISA; IFAT. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/142 |
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| van der Poel,W.H.M.; Pavio,N.; van der Goot,J.; van Es,M.; Martin,M.; Engel,B.. |
| Hepatitis E virus (HEV) is classified within the family Hepeviridae, genus Hepevirus. HEV genotype 3 (Gt3) infections are endemic in pigs in Western Europe and in North and South America and cause zoonotic infections in humans. Several serological assays to detect HEV antibodies in pigs have been developed, at first mainly based on HEV genotype 1 (Gt1) antigens. To develop a sensitive HEV Gt3 ELISA, a recombinant baculovirus expression product of HEV Gt3 open reading frame-2 was produced and coated onto polystyrene ELISA plates. After incubation of porcine sera, bound HEV antibodies were detected with anti-porcine anti-IgG and anti-IgM conjugates. For primary estimation of sensitivity and specificity of the assay, sets of sera were used from pigs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis E virus; Zoonosis; Swine; ELISA. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400334 |
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| Tayeb,IT; Nehme,P; Jaber,L; Barbour,EK. |
| The present study aims at developing an indirect ELISA to quantify yolk antibodies specific to all surface proteins of the invasive Salmonella Enteritidis (SE), which acquired the 1.8, 14.1, and ~ 50 Kb plasmids. An ELISA checkerboard was used in four different experiments to account for the different parameters included in the preliminary ELISA procedure, and consequently to maximize the difference in Optical Density (OD) values between control positive and negative yolk samples. The first experiment aimed at studying the impact of 5% Bovine Serum Albumin (BSA) dissolved in distilled water as a blocking reagent on a 28 µg/well SE antigen-coated plate, while applying the positive and negative control yolk samples to different concentrations of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antibodies; Egg yolk; ELISA; Salmonella Enteritidis. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000400009 |
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| Registros recuperados: 174 | |
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